styles - DO NOT REMOVE
Shared Resources
Biorepository and Tissue Technology
OVERVIEW
Availability of high-quality, annotated human tissue specimens and comprehensive histopathological services at the College of American Pathologists (CAP) - accredited UC San Diego Biorepository and Tissue Technology Shared Resources (BTTSR)
The BTTSR is founded on a well-established model that integrates tissue procurement, processing, storage, and multimodal (histologic, immunohistochemical, and molecular) analyses with traditional epidemiologic, clinicopathologic, and outcome parameters through a highly evolved bioinformatics system.
The mission of the BTTSR is to support MCC, C3, and outside researchers’ projects by providing annotated human bio- specimens, and state-of-the-art histology processing, interpretation, and histopathological analysis of samples.
Services
We provide a wide range of services
Biorepository Services
- Patient consenting
- Tissue acquisition
- Prospective collection
- Clinical trial support
- Tissue storage
- Distribution
- Blood derivatives: Buffy Coat, Plasma, Serum, PBMC
- Fresh Frozen Tissue: neoplastic and non-neoplastic
- Viable freeze
- Formalin fixed paraffin embedded Tissue: neoplastic& non-neoplastic
- Formalin fixed paraffin embedded Tissue: Autopsy
- PDX Models
- Other services
- DNA and RNA Extraction and QA
- Fill out a Request Form for Biorepository Services
Contact Information:
Sharmeela Kaushal
Biorepository Assistant Director
skaushal@health.ucsd.edu
Office: (858) 534-7302
Tissue Technology Services
- Routine Histology
- Tissue processing and sectioning (formalin fixed and frozen tissue)
- Routine H&E and specialized staining
- Immunohistochemistry/Immunofluorescence
- Antibody optimization and validation
- Single and multiplexing detection assays
- Whole slide scanning
- Brightfield AT2 Aperio
- Fluorescent (up to 6 channels + DAPI) PhenoImager Akoya
- Phenocycler Fusion: Ultra-plex Imaging
- Customized Tissue Micro Array (TMA)
- Experimental pathology consultation
- Click here to request services: https://redcap.link/TTSRRequestForm-HistologyServices
Contact Information:
M. Valeria Estrada
Tissue Technology Assistant Director
mvestrada@health.ucsd.edu
Office: (858) 534-8607
Recharge Rates
Cancer Center Council (C3) recharge rates: Cancer menter member internal rate plus 16% indirect cost.
| Item Description | MCC | Associate | Non-Member UCSD Faculty | Non-University | Cancer Center Council (C3) |
|---|---|---|---|---|---|
| Biorepository | |||||
| Ascites (Fresh) / Other Bodily Fluids | $55.00 | $72.00 | $72.00 | $132.00 | $63.80 |
| Ascites Processing | $70.00 | $91.00 | $91.00 | $167.00 | $81.20 |
| Block Storage / year | $7.00 | $9.00 | $9.00 | $16.00 | $8.12 |
| Buffy Coat (250 uL) | $39.00 | $51.00 | $51.00 | $93.00 | $45.24 |
| Buffy Coat Processing | $70.00 | $91.00 | $91.00 | $167.00 | $81.20 |
| DNA or RNA Extraction (All Tissue Types) | $49.00 | $64.00 | $64.00 | $118.00 | $56.84 |
| Paraffin Block Access | $75.00 | $98.00 | $98.00 | $180.00 | $87.00 |
| PBMC (1 mL) | $55.00 | $72.00 | $72.00 | $133.00 | $63.80 |
| PBMC Processing | $135.00 | $176.00 | $176.00 | $323.00 | $156.60 |
| Plasma or Serum (500 uL) | $11.00 | $14.00 | $14.00 | $26.00 | $12.76 |
| Plasma or Serum Processing | $70.00 | $91.00 | $91.00 | $168.00 | $81.20 |
| RNA/DNA + QA for Omics/Assay (12 Samples) | $115.00 | $150.00 | $150.00 | $276.00 | $130.40 |
| Saliva | $40.00 | $52.00 | $52.00 | $96.00 | $46.40 |
| Solid Fresh Frozen Tissue (50 mg) | $95.00 | $124.00 | $124.00 | $228.00 | $110.20 |
| Solid Fresh Tissue Collection (Biopsy - FFPE Processing) | $7.00 | $9.00 | $9.00 | $17.00 | $8.12 |
| Solid Fresh Tissue Collection (Biopsy - OCT Processing) | $70.00 | $91.00 | $91.00 | $168.00 | $81.20 |
| Solid Fresh Tissue Collection (Biopsy) | $120.00 | $156.00 | $156.00 | $286.00 | $139.20 |
| Solid Fresh Tissue Collection (Surgical) | $274.00 | $356.00 | $356.00 | $654.00 | $317.84 |
| Storage - Liquid (-80°) / per month, per Freezer | $100.00 | $130.00 | $130.00 | $238.00 | $34.80 |
| Storage - Liquid (-80°) / per month, per Shelf | $30.00 | $39.00 | $39.00 | $71.00 | $116.00 |
| Storage - Liquid (-80°) or LN2 per month | $20.00 | $26.00 | $26.00 | $48.00 | $23.20 |
| TMA - Case Identification | $74.00 | $96.00 | $96.00 | $176.00 | $85.84 |
| TMA Unstained slides/24 core TMA/Slide | $50.00 | $65.00 | $65.00 | $120.00 | $58.00 |
| TMA Unstained slides/48 core TMA/Slide | $70.00 | $91.00 | $91.00 | $166.00 | $81.20 |
| Urine (500 uL) | $11.00 | $14.00 | $14.00 | $27.00 | $12.76 |
| Viable Fresh Frozen Tissue | $308.00 | $400.00 | $400.00 | $734.00 | $357.28 |
| Whole Blood/Case | $81.00 | $105.00 | $105.00 | $193.00 | $93.96 |
| Xenografted Viable Freeze Tumor (PDX) | $150.00 | $195.00 | $195.00 | $357.00 | $174.00 |
| Services | |||||
| Assay Handling (hourly) | $50.00 | $65.00 | $65.00 | $120.00 | $58.00 |
| IRB Usage & Preparation (one-time) | $812.00 | $1,056.00 | $1,056.00 | $1,936.00 | $941.92 |
| Miscellaneous (per unit) | $100.00 | $130.00 | $130.00 | $238.00 | $116.00 |
| Pathologist's Assistant (hourly) | $150.00 | $195.00 | $195.00 | $358.00 | $174.00 |
| Pathology Consultation (hourly) | $285.00 | $371.00 | $371.00 | $680.00 | $330.60 |
| Rush Processing Fee (per unit) | $100.00 | $130.00 | $130.00 | $239.00 | $116.00 |
| SOP Development (one-time) | $247.00 | $316.00 | $316.00 | $580.00 | $286.52 |
| Tech Time (hourly) | $120.00 | $156.00 | $156.00 | $286.00 | $139.20 |
| Tissue Technology | |||||
| Grossing (per cassette) | $3.00 | $3.00 | $3.00 | $6.00 | $3.48 |
| Slide/Cassette Labeling | $1.00 | $1.00 | $1.00 | $2.00 | $1.16 |
| Frozen Tissue, Embedding | $7.00 | $9.00 | $9.00 | $17.00 | $8.12 |
| Frozen Section | $5.00 | $7.00 | $7.00 | $13.00 | $5.80 |
| Paraffin Block | $6.00 | $7.00 | $7.00 | $12.00 | $6.96 |
| Paraffin Section | $5.00 | $7.00 | $7.00 | $14.00 | $5.80 |
| Routine Stain (H&E) | $4.00 | $5.00 | $5.00 | $9.00 | $4.64 |
| Special Stain | $15.00 | $20.00 | $20.00 | $37.00 | $17.40 |
| Scrolls (up to 3 Scrolls at 10-20 um) | $10.00 | $13.00 | $13.00 | $25.00 | $11.60 |
| Aperio (BF) Scanning (20X) | $10.00 | $13.00 | $13.00 | $24.00 | $11.60 |
| Aperio (BF) Scanning (40X) | $15.00 | $20.00 | $20.00 | $37.00 | $17.40 |
| IF Scanning (20X) | $30.00 | $39.00 | $39.00 | $72.00 | $34.80 |
| IF Scanning (40X) | $40.00 | $52.00 | $52.00 | $95.00 | $46.40 |
| IHC Optimization | $195.00 | $254.00 | $254.00 | $465.00 | $226.20 |
| IHC/IF Staining (Primary Antibody not included) | $30.00 | $39.00 | $39.00 | $72.00 | $34.80 |
| Multiplexing IF (2 markers + DAPI) | $60.00 | $78.00 | $78.00 | $144.00 | $69.69 |
| Multiplexing IF (3 markers + DAPI) | $90.00 | $117.00 | $117.00 | $214.00 | $104.40 |
| Multiplexing IF (4 markers + DAPI) | $120.00 | $156.00 | $156.00 | $287.00 | $139.20 |
| Multiplexing IF (5 markers + DAPI) | $150.00 | $195.00 | $195.00 | $357.00 | $174.00 |
| Multiplexing IF (6 markers + DAPI) | $180.00 | $234.00 | $234.00 | $429.00 | $208.80 |
| Phenocycler Assay - Base Cost (Antibodies Not Included) | Market | Market | Market | Market | Market |
| Decalcification | $10.00 | $13.00 | $13.00 | $19.00 | $11.60 |
| TMA / Core | $5.00 | $7.00 | $7.00 | $14.00 | $5.80 |
| Consumables | |||||
| Antibody Aliquot/Slide (per unit) | $12.00 | $12.00 | $12.00 | $18.00 | $13.92 |
| Cassettes (per unit) | $1.00 | $1.00 | $1.00 | $1.50 | $1.16 |
| Cardboard box (per unit) | $2.00 | $2.00 | $2.00 | $3.00 | $1.16 |
| Large Slide Box (per unit) | $10.00 | $10.00 | $10.00 | $15.00 | $11.60 |
| Small Slide Box (per unit) | $6.75 | $6.75 | $6.75 | $10.50 | $7.83 |