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Shared Resources

Biorepository and Tissue Technology

OVERVIEW

Availability of high-quality, annotated human tissue specimens and comprehensive histopathological services at  the College of American Pathologists (CAP) - accredited UC San Diego Biorepository and Tissue Technology Shared Resources (BTTSR)

The BTTSR is founded on a well-established model that integrates tissue procurement, processing, storage, and  multimodal (histologic, immunohistochemical, and molecular) analyses with traditional epidemiologic, clinicopathologic,  and outcome parameters through a highly evolved bioinformatics system.  

The mission of the BTTSR is to support MCC, C3, and outside researchers’ projects by providing annotated human bio- specimens, and state-of-the-art histology processing, interpretation, and histopathological analysis of samples. 


 

Services

We provide a wide range of services

Biorepository Services

  • Patient consenting​
  • Tissue acquisition​
    • Prospective collection ​
    • Clinical trial support​
  • Tissue storage ​
  • Distribution ​
    • Blood derivatives: Buffy Coat, Plasma, Serum, PBMC ​
    • Fresh Frozen Tissue: neoplastic and non-neoplastic ​
    • Viable freeze  ​
    • Formalin fixed paraffin embedded  Tissue: neoplastic& non-neoplastic ​
    • Formalin fixed paraffin embedded  Tissue: Autopsy ​
    • PDX Models ​
  • Other services ​
    • DNA and RNA Extraction and QA 
  • Fill out a Request Form for Biorepository Services

Contact Information:

Sharmeela Kaushal
Biorepository Assistant Director
skaushal@health.ucsd.edu
Office: (858) 534-7302

Tissue Technology Services

  • Routine Histology​
    • Tissue processing and sectioning  (formalin fixed and frozen tissue)​
    • Routine H&E and specialized staining ​
  • Immunohistochemistry/Immunofluorescence​
    • Antibody optimization and validation​
    • Single and multiplexing detection assays ​
  • Whole slide scanning​
    • Brightfield AT2 Aperio​
    • Fluorescent (up to 6 channels + DAPI) PhenoImager Akoya​
  • Phenocycler Fusion: Ultra-plex Imaging​
  • Customized Tissue Micro Array (TMA) ​
  • Experimental pathology consultation​​
  • Click here to request services: https://redcap.link/TTSRRequestForm-HistologyServices

Contact Information:​

M. Valeria Estrada​
Tissue Technology Assistant Director​
mvestrada@health.ucsd.edu

Office: (858) 534-8607

Recharge Rates

​Item Description

Measurable Unit

MCC Member Cost

C3 Cost

UCSD (Non-MCC Member) Cost

Industry Rate

Ascites (Fresh) / Other Bodily Fluids

per case

$55

$58

$72

$132

Assay Handling

per hour

$50

$53

$65

$120

Block Storage / year

per block

$7

$7

$9

$16

Buffy Coat (250 uL)

per aliquot

$39

$41

$51

$93

Cell Count Assay / sample

per cassette

$4

$4

$5

$9

DNA or RNA Extraction (All Tissue Types)

per sample

$49

$52

$64

$118

Frozen Section

per section

$5

$5

$7

$13

Frozen Tissue, Embedding

per OCT block

$7

$7

$9

$17

IHC Optimization

per antibody

$195

$207

$254

$465

IRB Usage & Preparation/One-Time/PI

each

$812

$861

$1,056

$1,936

Miscellaneous

per unit

$100

$106

$130

$238

Paraffin Block

per block

$6

$6

$7

$12

Paraffin Section

per slide

$5

$5

$7

$14

Pathology Consultation Fee

per hour

$285

$302

$371

$680

PBMC (1 mL)

per aliquot

$55

$58

$72

$133

Plasma or Serum (500 uL)

per aliquot

$11

$12

$14

$26

RNA/DNA + QA for Omics/Assay (12 Samples)

per cassette

$115

$122

$150

$276

Routine Stain

per slide

$4

$4

$5

$9

Saliva

each

$40

$42

$52

$96

SOP Development

each

$247

$262

$316

$580

Special Stain

per slide

$15

$16

$20

$37

Storage - Liquid (-80º) or LN2 per month

per box

$20

$21

$26

$48

Tech Time

per hour

$120

$127

$156

$286

Tissue Print/Aliquot

each

$170

$180

$221

$406

Urine (500 uL)

per aliquot

$11

$12

$14

$27

Viable Fresh Frozen Tissue

each

$308

$326

$400

$734

Whole Blood/Case

per tube

$81

$86

$105

$193

Xenografted Viable Freeze Tumor (PDX)

each

$150

$159

$195

$357

Antibody Aliquot/Slide

each

$5

$5

$7

$13

Aperio Scanning (20X)

per slide

$10

$11

$13

$24

Aperio Scanning (40X)

per slide

$15

$16

$20

$37

Ascites Processing

per tube

$70

$74

$91

$167

Buffy Coat Processing

per tube

$70

$74

$91

$167

Cassettes

per 10 units

$10

$11

$10

$18

Cassette Labeling/Grossing

per cassette

$3

$3

$3

$6

Cytospin Use

per slide

$4

$4

$5

$9

Decalcification

per order

$10

$11

$10

$19

Delivery Fee

per round trip

$12

$13

$12

$21

IF Scanning (20X)

per slide

$30

$32

$39

$72

IF Scanning (40X)

per slide

$40

$42

$52

$95

IHC/IF Staining (Primary Antibody not included)

per slide

$30

$32

$39

$72

Large Slide Box

per unit

$7

$7

$9

$17

Multiplexing IF (2 markers + DAPI)

per slide

$60

$64

$78

$144

Multiplexing IF (3 markers + DAPI)

per slide

$90

$95

$117

$214

Multiplexing IF (4 markers + DAPI)

per slide

$120

$127

$156

$287

Multiplexing IF (5 markers + DAPI)

per slide

$150

$159

$195

$357

Multiplexing IF (6 markers + DAPI)

per slide

$180

$191

$234

$429

Paraffin Block Access

per block

$75

$80

$98

$180

Pathologist's Assistant Time

per hour

$150

$159

$195

$358

PBMC Processing

per tube

$135

$143

$176

$323

Phenocycler Assay - Base Cost (Antibodies not included)

per slide

Varies based on project and market price

Varies based on project and market price

Varies based on project and market price

Varies based on project and market price

Plasma or Serum Processing

per tube

$70

$74

$91

$168

Rush Processing Fee

per unit

$100

$106

$130

$239

Scrolls (3 scrolls at 10-20 um)

per block

$10

$11

$13

$25

Slide Labeling

per slide

$1

$1

$1

$2

Small Slide Box

per unit

$5

$5

$5

$10

Solid Fresh Frozen Tissue (50 mg)

per aliquot

$95

$101

$124

$228

Solid Fresh Tissue Collection (Biopsy - FFPE Processing)

per block

$7

$7

$9

$17

Solid Fresh Tissue Collection (Biopsy - OCT Processing)

per block

$70

$74

$91

$168

Solid Fresh Tissue Collection (Biopsy)

each

$120

$127

$156

$286

Solid Fresh Tissue Collection (Surgical)

each

$274

$290

$356

$654

Storage - Liquid (-80º) / per month, per Shelf

per shelf

$30

$32

$39

$71

Storage - Liquid (-80º) / per month, per Freezer

per freezer

$100

$106

$130

$238

TMA / Core

each

$5

$5

$7

$14

TMA - Case Identification

per hour

$74

$78

$96

$176

TMA Unstained slides/24 core TMA/Slide

per slide

$50

$53

$65

$120

TMA Unstained slides/48 core TMA/Slide

per slide

$70

$74

$91

$166