Shared Resources
Biorepository and Tissue Technology
OVERVIEW
Availability of high-quality, annotated human tissue specimens and comprehensive histopathological services at the College of American Pathologists (CAP) - accredited UC San Diego Biorepository and Tissue Technology Shared Resources (BTTSR)
The BTTSR is founded on a well-established model that integrates tissue procurement, processing, storage, and multimodal (histologic, immunohistochemical, and molecular) analyses with traditional epidemiologic, clinicopathologic, and outcome parameters through a highly evolved bioinformatics system.
The mission of the BTTSR is to support MCC, C3, and outside researchers’ projects by providing annotated human bio- specimens, and state-of-the-art histology processing, interpretation, and histopathological analysis of samples.
Services
We provide a wide range of services
Biorepository Services
- Patient consenting
- Tissue acquisition
- Prospective collection
- Clinical trial support
- Tissue storage
- Distribution
- Blood derivatives: Buffy Coat, Plasma, Serum, PBMC
- Fresh Frozen Tissue: neoplastic and non-neoplastic
- Viable freeze
- Formalin fixed paraffin embedded Tissue: neoplastic& non-neoplastic
- Formalin fixed paraffin embedded Tissue: Autopsy
- PDX Models
- Other services
- DNA and RNA Extraction and QA
- Fill out a Request Form for Biorepository Services
Contact Information:
Sharmeela Kaushal
Biorepository Assistant Director
skaushal@health.ucsd.edu
Office: (858) 534-7302
Tissue Technology Services
- Routine Histology
- Tissue processing and sectioning (formalin fixed and frozen tissue)
- Routine H&E and specialized staining
- Immunohistochemistry/Immunofluorescence
- Antibody optimization and validation
- Single and multiplexing detection assays
- Whole slide scanning
- Brightfield AT2 Aperio
- Fluorescent (up to 6 channels + DAPI) PhenoImager Akoya
- Phenocycler Fusion: Ultra-plex Imaging
- Customized Tissue Micro Array (TMA)
- Experimental pathology consultation
- Click here to request services: https://redcap.link/TTSRRequestForm-HistologyServices
Contact Information:
M. Valeria Estrada
Tissue Technology Assistant Director
mvestrada@health.ucsd.edu
Office: (858) 534-8607
Recharge Rates
Item Description |
Measurable Unit |
MCC Member Cost |
C3 Cost |
UCSD (Non-MCC Member) Cost |
Industry Rate |
---|---|---|---|---|---|
Ascites (Fresh) |
per case |
$55 |
$64 |
$72 |
$123 |
Assay Handling |
per hour |
$50 |
$58 |
$65 |
$110 |
Block Storage / year |
per block |
$7 |
$8 |
$9 |
$14 |
Buffy Coat (250 uL) |
per aliquot |
$39 |
$46 |
$51 |
$87 |
Cell Count Assay / sample |
per cassette |
$4 |
$5 |
$5 |
$8 |
DNA or RNA Extraction (All Tissue Types) |
per sample |
$49 |
$57 |
$64 |
$109 |
Frozen Section |
per section |
$5 |
$6 |
$7 |
$11 |
Frozen Tissue, Embedding |
per OCT block |
$7 |
$8 |
$9 |
$15 |
IHC Optimization |
per antibody |
$195 |
$226 |
$254 |
$431 |
IRB Usage & Preparation/One-Time/PI |
each |
$812 |
$942 |
$1,056 |
$1,789 |
Miscellaneous |
per unit |
$100 |
$116 |
$130 |
$221 |
Paraffin Block |
per block |
$6 |
$7 |
$7 |
$11 |
Paraffin Section |
per slide |
$5 |
$6 |
$7 |
$12 |
Pathology Consultation Fee |
per hour |
$285 |
$330 |
$371 |
$628 |
PBMC (1 mL) |
per aliquot |
$55 |
$63 |
$72 |
$121 |
Plasma or Serum (500 uL) |
per aliquot |
$11 |
$13 |
$14 |
$24 |
RNA/DNA + QA for Omics/Assay (12 Samples) |
per cassette |
$115 |
$133 |
$150 |
$254 |
Routine Stain |
per slide |
$4 |
$5 |
$5 |
$8 |
Saliva |
each |
$40 |
$47 |
$52 |
$89 |
SOP Development |
each |
$525 |
$608 |
$683 |
$1,157 |
Special Stain |
per slide |
$15 |
$18 |
$20 |
$34 |
Storage - Liquid (-80º) or LN2 per month |
per box |
$20 |
$23 |
$26 |
$45 |
Tech Time |
per hour |
$120 |
$139 |
$156 |
$264 |
Tissue Print/Aliquot |
each |
$170 |
$197 |
$221 |
$374 |
Urine (500 uL) |
per aliquot |
$11 |
$13 |
$14 |
$23 |
Viable Fresh Frozen Tissue |
each |
$308 |
$357 |
$400 |
$677 |
Whole Blood/Case |
per tube |
$81 |
$94 |
$105 |
$179 |
Xenografted Viable Freeze Tumor (PDX) |
each |
$150 |
$174 |
$195 |
$330 |
Antibody Aliquot/Slide |
each |
$5 |
$6 |
$7 |
$12 |
Aperio Scanning (20X) |
per slide |
$10 |
$12 |
$13 |
$22 |
Aperio Scanning (40X) |
per slide |
$15 |
$18 |
$20 |
$33 |
Ascites Processing |
per tube |
$70 |
$81 |
$91 |
$154 |
Buffy Coat Processing |
per tube |
$70 |
$81 |
$91 |
$155 |
Cassettes |
per 10 units |
$10 |
$12 |
$10 |
$17 |
Cassette Labeling/Grossing |
per cassette |
$3 |
$3 |
$3 |
$6 |
Cytospin Use |
per slide |
$4 |
$4 |
$5 |
$8 |
Decalcification |
per order |
$10 |
$12 |
$10 |
$18 |
Delivery Fee |
per round trip |
$12 |
$14 |
$12 |
$21 |
IF Scanning (20X) |
per slide |
$30 |
$35 |
$39 |
$66 |
IF Scanning (40X) |
per slide |
$40 |
$46 |
$52 |
$87 |
IHC/IF Staining (Primary Antibody not included) |
per slide |
$30 |
$35 |
$39 |
$66 |
Large Slide Box |
per unit |
$7 |
$9 |
$9 |
$15 |
Multiplexing IF (2 markers + DAPI) |
per slide |
$60 |
$69 |
$78 |
$133 |
Multiplexing IF (3 markers + DAPI) |
per slide |
$90 |
$1014 |
$117 |
$197 |
Multiplexing IF (4 markers + DAPI) |
per slide |
$120 |
$139 |
$156 |
$264 |
Multiplexing IF (5 markers + DAPI) |
per slide |
$150 |
$174 |
$195 |
$330 |
Multiplexing IF (6 markers + DAPI) |
per slide |
$180 |
$208 |
$234 |
$396 |
Paraffin Block Access |
per block |
$75 |
$87 |
$98 |
$166 |
Pathologist's Assistant Time |
per hour |
$150 |
$174 |
$195 |
$331 |
PBMC Processing |
per tube |
$135 |
$157 |
$176 |
$299 |
Phenocycler Assay - Base Cost (Antibodies not included) |
per slide |
$2,000 |
$2,319 |
$2,000 |
$3,387 |
Plasma or Serum Processing |
per tube |
$70 |
$81 |
$91 |
$154 |
Rush Processing Fee |
per unit |
$100 |
$116 |
$130 |
$220 |
Scrolls (3 scrolls at 10-20 um) |
per block |
$10 |
$12 |
$13 |
$22 |
Slide Labeling |
per slide |
$1 |
$1 |
$1 |
$1 |
Small Slide Box |
per unit |
$5 |
$5 |
$5 |
$5 |
Solid Fresh Frozen Tissue (50 mg) |
per aliquot |
$95 |
$110 |
$124 |
$210 |
Solid Fresh Tissue Collection (Biopsy - FFPE Processing) |
per block |
$7 |
$8 |
$9 |
$16 |
Solid Fresh Tissue Collection (Biopsy - OCT Processing) |
per block |
$70 |
$81 |
$91 |
$154 |
Solid Fresh Tissue Collection (Biopsy) |
each |
$120 |
$139 |
$156 |
$265 |
Solid Fresh Tissue Collection (Surgical) |
each |
$274 |
$318 |
$356 |
$602 |
Storage - Liquid (-80º) / per month, per Shelf |
per shelf |
$30 |
$34 |
$39 |
$65 |
Storage - Liquid (-80º) / per month, per Freezer |
per freezer |
$100 |
$116 |
$130 |
$220 |
TMA / Core |
each |
$5 |
$6 |
$7 |
$12 |
TMA - Case Identification |
per hour |
$74 |
$85 |
$95 |
$160 |
TMA Unstained slides/24 core TMA/Slide |
per slide |
$50 |
$58 |
$65 |
$110 |
TMA Unstained slides/48 core TMA/Slide |
per slide |
$70 |
$82 |
$91 |
$155 |