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Shared Resources

Transgenic Mouse

OVERVIEW

The Transgenic Mouse shared resource is a state-of-the-art facility that has an outstanding track record in the production of genetically altered mice. Transgenic mice carrying new or novel genes are created by microinjection of RNA and/or DNA into the pronuclei of fertilized eggs. Knock-out mice lacking specific genes of interest are created by homologous recombination in embryonic stem cells followed by injection into blastocysts to create chimeric mice. Highly experienced personnel produce transgenic, CRISPR, and knock-out mice for UC San Diego investigators at very reasonable cost and with very short lead times. 

 

Transgenic Mouse Shared Resource Services

  • CRISPR cytoplasmic or pronuclear injection of RNA/DNA to create transgenic mice
  • Pronuclear microinjection of DNA to create transgenic mice
  • Blastocyst microinjection of embryonic stem cells to create knockout mice.
  • Embryonic stem cell gene targeting for homologous recombination
  • Embryo rederivation to generate pathogen-free mice
  • Ovary transplantation to rescue infertile mice
  • Embryo Freezing to archive existing mouse lines
  • In Vitro Fertilization with frozen or fresh sperm to regenerate mouse lines
  • Sperm Freezing to archive existing mouse lines
  • Embryo thawing to regenerate mouse lines

Service Details

Request services

Register to use services


NOTE: Information from non-Moores Cancer Center principal investigators must be on file before utilizing Shared Resource facilities.

Recharge Rates

Details for each of the following services, can be read here.

Service MCC rate
members		 UC rate Non-UC rate
CRISPR – cytoplasmic or pronuclear injections $ 2,970 $ 3,300 $ 4,785
Pronuclear Injection - Inbred Strain $ 4,263 $ 4,737 $ 6,869
Blastocyst Injection - price per clone $ 1,985 $ 2,205 $ 3,198
Commercially purchased ES cell maintenance
* (please click the link above for detailed billing facts)		 $ 541 $ 601 $ 872
ES-cell Gene Targeting
(Knock-Out or Knock-In) into129 ES cells		 $ 3,898 $ 4,323 $ 6,268
ES-cell Gene Targeting
(Knock-Out or Knock-In) into C57Bl6 ES cells		 $ 5,241 $ 5,823 $ 8,444
RMCE Electroporation $ 2,657 $ 2,952 $ 4,280
Chromosome Count $ 162 $ 180 $ 261
Embryo Rederivation $ 1,439 $ 1,599 $ 2,319
Ovary transplant $ 936 $ 1,020 $ 1,479
Embryo Freezing $ 1,584 $ 1,685 $ 2,444
In- vitro Fertilization (IVF) $ 2,190 $ 2,433 $ 3,528
Sperm Freezing*
(please click the link above for detailed billing facts)		 $ 314 $ 349 $ 505
Embryo Thawing
(price per line being thawed)		 $ 942 $ 1,048 $ 1,519
Tailing for screening
(price per transgenic job)		 $ 225 $ 250 $ 363

  

Where do I start?

So you want to make a transgenic or knockout line? Here's what you'll need to have before we can begin work on your project:

  • Approved animal protocol in place
  • DNA or RNA prepared using protocols provided by Core
  • Plan in place for screening ES cells (for KO)
  • Assigned animal space as destination for mice
  • Plan in place for screening pups
  • Trained personnel to breed mice, cut tails, and screen DNA
  • UC San Diego index number or an off-campus purchase order
UC San Diego Transgenic Core Request For Services

If you require a support letter from the Transgenic Core for your grant submissions, please contact MellonAdmin@ucsd.edu.

Contact

Director:
Pamela Mellon, PhD
Professor of Obstetrics, Gynecology and Reproductive Sciences and Neuroscience 

Co-Director: 
Miles Wilkinson
Professor of Obstetrics, Gynecology and Reproductive Science

Contacts:
Jun Zhao
juzhao@ucsd.edu
Sang Lee
srl001@health.ucsd.edu 

Location: 
UC San Diego 
Cellular and Molecular Medicine
9500 Gilman Drive
La Jolla, CA 92093-0674