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Shared Resources

Transgenic and CRISPR Mouse

OVERVIEW

The Transgenic and CRISPR Mouse shared resource is a state-of-the-art facility that has an outstanding track record in the production of genetically altered mice. Mice carrying altered, new or novel genes are created by microinjection of RNA and/or DNA into fertilized mouse eggs. Knock-out mice lacking specific genes of interest are created by homologous recombination in embryonic stem cells followed by injection into blastocysts to create chimeric mice. Highly experienced personnel produce transgenic, CRISPR edited, and knock-out mice for UC San Diego investigators at reasonable cost and with short lead times.  

 

Transgenic Mouse Shared Resource Services

  • CRISPR cytoplasmic or pronuclear injection of RNA/DNA to create genetically modified mice
  • Pronuclear microinjection of DNA to create transgenic mice
  • Blastocyst microinjection of embryonic stem cells to create knockout mice.
  • Embryonic stem cell gene targeting for homologous recombination
  • Embryo rederivation to generate pathogen-free mice
  • Embryo freezing to archive existing mouse lines
  • In Vitro fertilization with frozen or fresh sperm to regenerate mouse lines
  • Sperm freezing to archive existing mouse lines
  • Embryo thawing to regenerate mouse lines
  • Consultation on CRISPR design

Service Details

Request services

Register to use services


NOTE: Information from non-Moores Cancer Center principal investigators must be on file before utilizing Shared Resource facilities.

Recharge Rates

Details for each of the following services can be read here. Price updates are effective 9/1/23.

Service MCC rate
members		 UC rate Non-UC rate
CRISPR – cytoplasmic or pronuclear injections $ 3,564 $ 3,960 $ 6,708
Pronuclear Injection $ 5,116 $ 5,684 $ 9,629
Blastocyst Injection - price per clone $ 2,382 $ 2,646 $ 4,482
Commercially purchased ES cell maintenance*
(please click the link for detailed billing facts)		 $ 649 $ 721 $ 1,221
ES-cell Gene Targeting
(Knock-Out or Knock-In) into 129 ES cells		 $ 4,678 $ 5,188 $ 8,788
ES-cell Gene Targeting
(Knock-Out or Knock-In) into C57Bl6 ES cells		 $ 6,289 $ 6,988 $ 11,838
Chromosome Count $ 194 $ 216 $ 366
Embryo Rederivation $ 1,727 $ 1,919 $ 3,251
Embryo Freezing $ 1,901 $ 2,022 $ 3,425
In Vitro Fertilization (IVF) $ 2,628 $ 2,920 $ 4,946
Sperm Freezing*
(please click the link for detailed billing facts)		 $ 377 $ 419 $ 710
Embryo Thawing
(price per line being thawed)		 $ 1,130 $ 1,258 $ 2,131
Consultation for CRISPR Design - per hour $ 85 $ 94 $ 158

Request services  

Where do I start?

So you want to make a transgenic or knockout line? Here's what you'll need to have before we can begin work on your project:

  • Approved animal protocol in place
  • DNA or RNA prepared using protocols provided by Core
  • Plan in place for screening ES cells (for KO)
  • Assigned animal space as destination for mice
  • Plan in place for screening pups
  • Trained personnel to breed mice, cut tails, and screen DNA
  • UC San Diego Chart String or an off-campus purchase order
UC San Diego Transgenic Core Request For Services

If you require a support letter from the Transgenic Core for your grant submissions, please contact mfwilkinson@health.ucsd.edu or pmellon@health.ucsd.edu.

Contacts

Director:
Pamela Mellon, PhD
Professor of Obstetrics, Gynecology and Reproductive Sciences and Neuroscience 

Co-Director: 
Miles Wilkinson, PhD
Professor of Obstetrics, Gynecology and Reproductive Science

Contacts:
Sang Lee, PhD
srl001@health.ucsd.edu
Ryan Fiske, BS
rfiske@health.ucsd.edu 

Location: 
UC San Diego 
Cellular and Molecular Medicine
East Building, First Floor, 1061
9500 Gilman Drive
La Jolla, CA 92093-0674