Shared Resources
Transgenic Mouse
OVERVIEW
The Transgenic Mouse shared resource is a state-of-the-art facility that has an outstanding track record in the production of genetically altered mice. Transgenic mice carrying new or novel genes are created by microinjection of RNA and/or DNA into the pronuclei of fertilized eggs. Knock-out mice lacking specific genes of interest are created by homologous recombination in embryonic stem cells followed by injection into blastocysts to create chimeric mice. Highly experienced personnel produce transgenic, CRISPR, and knock-out mice for UC San Diego investigators at very reasonable cost and with very short lead times.
Transgenic Mouse Shared Resource Services
- CRISPR cytoplasmic or pronuclear injection of RNA/DNA to create transgenic mice
- Pronuclear microinjection of DNA to create transgenic mice
- Blastocyst microinjection of embryonic stem cells to create knockout mice.
- Embryonic stem cell gene targeting for homologous recombination
- Embryo rederivation to generate pathogen-free mice
- Ovary transplantation to rescue infertile mice
- Embryo Freezing to archive existing mouse lines
- In Vitro Fertilization with frozen or fresh sperm to regenerate mouse lines
- Sperm Freezing to archive existing mouse lines
- Embryo thawing to regenerate mouse lines
NOTE: Information from non-Moores Cancer Center principal investigators must be on file before utilizing Shared Resource facilities.
Recharge Rates
Details for each of the following services, can be read here.
Service | MCC rate members |
UC rate | Non-UC rate |
CRISPR – cytoplasmic or pronuclear injections | $ 2,970 | $ 3,300 | $ 4,785 |
Pronuclear Injection - Inbred Strain | $ 4,263 | $ 4,737 | $ 6,869 |
Blastocyst Injection - price per clone | $ 1,985 | $ 2,205 | $ 3,198 |
Commercially purchased ES cell maintenance * (please click the link above for detailed billing facts) |
$ 541 | $ 601 | $ 872 |
ES-cell Gene Targeting (Knock-Out or Knock-In) into129 ES cells |
$ 3,898 | $ 4,323 | $ 6,268 |
ES-cell Gene Targeting (Knock-Out or Knock-In) into C57Bl6 ES cells |
$ 5,241 | $ 5,823 | $ 8,444 |
RMCE Electroporation | $ 2,657 | $ 2,952 | $ 4,280 |
Chromosome Count | $ 162 | $ 180 | $ 261 |
Embryo Rederivation | $ 1,439 | $ 1,599 | $ 2,319 |
Ovary transplant | $ 936 | $ 1,020 | $ 1,479 |
Embryo Freezing | $ 1,584 | $ 1,685 | $ 2,444 |
In- vitro Fertilization (IVF) | $ 2,190 | $ 2,433 | $ 3,528 |
Sperm Freezing* (please click the link above for detailed billing facts) |
$ 314 | $ 349 | $ 505 |
Embryo Thawing (price per line being thawed) |
$ 942 | $ 1,048 | $ 1,519 |
Tailing for screening (price per transgenic job) |
$ 225 | $ 250 | $ 363 |
Where do I start?
So you want to make a transgenic or knockout line? Here's what you'll need to have before we can begin work on your project:
- Approved animal protocol in place
- DNA or RNA prepared using protocols provided by Core
- Plan in place for screening ES cells (for KO)
- Assigned animal space as destination for mice
- Plan in place for screening pups
- Trained personnel to breed mice, cut tails, and screen DNA
- UC San Diego index number or an off-campus purchase order
If you require a support letter from the Transgenic Core for your grant submissions, please contact MellonAdmin@ucsd.edu.
Helpful Links
UC San Diego links
Molecular genetics and mouse biology resources
- Mouse Genome Database
- National Center for Biotechnology Information
- The Virtual Embryo
- Andras Nagy's Lab at the Mount Sinai Simon Lunenfeld Research Institute - Cre Transgenic Database
- Whole Mouse Catalog
- International Gene Trap Consortium (represents all publicly available gene trap cell lines which are available on a non-collaborative basis for a nominal handling fee)
- Ensembl Project (provides automated genome annotation with a focus on chordate organisms)
Vendors
- Harlan
- Charles River Laboratories, Inc.
- Taconic
- The Jackson Laboratory
- Marshall BioResources
- Incyte Genomics (formerly Genome Systems, Inc.)
E-mail lists
Contact
Director:
Pamela Mellon, PhD
Professor of Obstetrics, Gynecology and Reproductive Sciences and Neuroscience
Co-Director:
Miles Wilkinson
Professor of Obstetrics, Gynecology and Reproductive Science
Contacts:
Jun Zhao
juzhao@ucsd.edu
Sang Lee
srl001@health.ucsd.edu
Location:
UC San Diego
Cellular and Molecular Medicine
9500 Gilman Drive
La Jolla, CA 92093-0674